Mouth epithelium

Project Etwinning 2009/10

Experiencies without frontiers!

Experience 1: Study of oral epithelium and oral bacteria. Microscopy of the bacteria in yogurt. (two days)

Objectives:

Make a histologic and microbiological preparation: sample collection, extension, fixation, staining, washing and observation.

Directly observe the cells and bacteria in our mouth as well as those found in yogurt with active ingredients.

Recognize certain cell types and types of bacterial associations.

Materials:

Slides and coverslips

Enmangada needle or inoculation loop

Petri dish or staining tank

Wash bottle

Lighter

Methylene blue

Microscope

Protocol:

Day 1: Preparation of biological samples to be displayed. To do this follow these steps:

1: Extraction of the sample with a fingernail scraping the inside of the cheeks (buccal epithelium) or by scraping with a toothpick and carefully the insertion of the teeth and the gum (bacteria) or picking up a goto yogurt.

2: Place one got water (better in saline) on a slide and place the matter that we have extracted above.

3: With the needle enmangada perform a flutter prior smear slide.

4: Perform air drying.

5: Perform the setting from the slide through the flame of a lighter and avoiding overheating.

6: Place the smear on the petri dish to make a simple stained with methylene blue, letting it act between one or two minutes.

7: Remove excess dye by washing.

8: Meet the coverslip on the sample.

Day 2: Observing the strong microscope with 40x objective.

Click on the picture to see the video:

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