Blood experiment

Protocol:

First day: Preparation of the extensions. These steps must be followed.
1. Extraction of capyllary not anticoagulated blood of a lateral face of the fleshy part of the third or fouth finger of one of the hands.
2. Place a small drop of blood approximately two centimeters away from the edge of de microscope slide.
3. We place the slide with the drop of blood on a table.
4. We grab another microscope slide with the forefingers and thumb of the right hand and lean it on the one that has the drop of blood in front of it. The angle between both slides has to be from 30 to 45 degrees.
5. Pull the slide back until it touches the drop of blood.
6. Let the drop spead through out the surface of contact of both slides.
7. Before the blood reaches the edges, slip slide forward with firm and uniform movement at avenage speed.
8. End up the displacement, approximately, one centimeters before the and, with on up word movement.
9. We quickly dry the extension, shaking up.
10. Once dried we will asses the extension with “methond” or ethonal covering it for three minutes.
11. Drain and let it dry.

Note: We rehearsed first with one drop water to ensure the technique.

Second day: Dyeing of “GIEMSA” and observation undar the microscope objetive of 40x and 100x. We take the following steps.
1. Dilution of dye of “GIMESACON 2 milliliters” of solution ph 7,2 in a test tube 0,2 milliliters.
2. Slowly mix in the tube and with a pipette “Pasteur” caver the “Frotis” for 25 minutes.
3. Wash and drain with water.
4. Wash with “Buffer” ph 7,2 until remains of the dye are eliminated.
5. Let it drain and dry vertically.
6. See the preparation trough the microscope.

Extension of blood.




1. Hold the slide.
2. Deposit blood.
3. Place the holder extensor.
4. Move the holder extensor.
5. Let the blood.
6. Slide the holder extensor.
7. Dry the extension.
8. Label the holder.

Click on the image to view video.




Activities.

1. As you can see in the video, we have done the blood film with the slide, because we consider it is the best method. Do you know any other process? What are the differences with the one we have used? What are the advantages and disadvantages?
2. What is to due the presence of streaks in a blood film? Can you draw the aspect on of a extension?
3. What are the cellular elements you can observe in the micrographs?
4. Why did the central part is the most pale?


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